René Handrick

René Handrick

Kontaktdaten

Tel.
+49 - (0) 7351 582-440
handrick@hochschule-bc.de

Adresse

Büro:
Haus PBT
P3.04

Hubertus-Liebrecht-Str. 35
88400 Biberach
Deutschland

Lebenslauf

Current position

Lab head at the Institute of Applied Biotechnology, Faculty of Biotechnology,

Lecturer / module coordinator at the master´s program Pharmaceutical Biotechnology

University of Applied Sciences Biberach

Hubertus–Liebrecht-Str. 35, 88400 Biberach

phone.: +49-7351-582-440/ -490,

fax: +49-7351-582-469

E-mail: rene.handrick@hochschule-bc.de



 

Scientific Career


1991 - 1997

Study of biology at the University of Göttingen (Diploma)

01/1996 - 01/1997

Diploma-thesis in the lab of Prof. Dr. Hans G. Schlegel/Dr. Dieter Jendrossek at the Institute of Microbiology and Genetics in Göttingen (Germany). Title of the thesis: 'Studies on intracellular degradation of poly(3-hydroxybutyrate) in bacteria'

03/1995 - 09/1995

Industrial projects, Sartorius AG

02/1997 - 09/1997

Research assistant at the Institute of Microbiology and Genetics, University of Göttingen. Industrial project WissTrans GmbH, Prof. Dr. G. Gottschalk

10/1997 - 05/2002

PhD-thesis in the lab of Prof. Dr. Dieter Jendrossek at the Institute of Microbiology and Genetics, University of Göttingen and at the Centre of Biochemical Engineering (Institute of Microbiology), University of Stuttgart (Germany). Title of the thesis: 'Novel poly(3-hydroxybutyrate) depolymerases from Paucimonas lemoignei and Rhodospirillum rubrum'. (summa cum laude)

06/2002 - 10/2002

Research assistant and postdoctoral research fellow in the lab of Prof. Dr. Dieter Jendrossek at the Centre of Biochemical Engineering (Institute of Microbiology), University of Stuttgart (Germany)

11/2002 - 10/2007

Postdoctoral research fellow in the lab of Prof. Dr. Claus Belka at the Department of Radiation Oncology/Experimental Radiation Oncology, Comprehensive Cancer Center (CCC) Tübingen, University of Tübingen (Germany)

10/2007 - 01/2009

Postdoctoral research fellow/Group leader in the lab of Prof. Dr. Verena Jendrossek at the Institute of Cell Biology (Cancer Research), Department of Molecular Cell Biology, University of Duisburg-Essen (Germany), Establishment of an S2 virus lab

Since 01/2009

Lecturer and scientist at the University of Applied Sciences, Biberach, and

Lab head at the Institute of Applied Biotechnology, Faculty of Biotechnology (former Faculty of Pharmaceutical Biotechnology), University of Applied Sciences, Biberach (Germany)

Working in public funded projects as well as in industrial projects with focus on cell line-, USP- and DSP- development

Since 03/2010

Training of Master´s students at the faculty of Biotechnology at University of Applied Biotechnology

Module responsibilities:

  • “Scientific project work Bioprocessing 1+2” (module coordinator),
  • “Bioprocessing”, ”Bioprocess technology/fermentation”



Scientific interests/Expertise:

Molecular microbial and cell biology, metabolism, cell signalling, target identification, apoptosis, cell death, bioproduction, non coding RNAs, miRNA, cell line development, evolution, post translational modifications (e.g. glycosylation) in pro- and eucaryotes, expression of soluble proteins and glycosylation in procaryotes and eukaryotes, bioprocessing, media development, New Biological Entities (NBEs), chemometics, M3C, PAT, automatization, sensor technology

 

Visiting Research Fellowship

09/1995

Interuniversity Institute for Marine Science, Eilat (Israel), Prof. A. Oren

12/1996

ETH Zürich, Zürich (Schwitzerland), Dr. Fischer, Prof. D. Seebach

09-10/1998

Laboratory of Molecular Biology der KINAGAWA UNIVERSITY, Kinagawa (Japan), Prof. T. Saito

11-12/2004

Max Delbrück Center for molecular Medicine MDC, Berlin (Germany), Prof. P. Daniel

11-12/2006 and 05/2008

University of Hohenheim, Stuttgart (Germany), LSC Service facility (Proteomics, DIGE, ESI-MS), Dr. J. Pfannstiel

07/2016

Laboratory for Metabolic Research, Dept. Biochemistry, Belarus State University, Minsk Belarus, Appl. Prof. Mikhail Sholukh




Scholarships and Awards

1997

Scholarship of the DFG Graduate school: “Chemical activities of microorganisms”

09/1998

Travel grant from the DFG Graduate school for residency at Kanagawa university (Japan)

01/1998 - 01/2000

Scholarship of the German National Merit Foundation

2006

Poster Price of the IZKF, Tübingen



Societies/Committees

Association for General and Applied Microbiology (VAAM)

European Society Animal Cell Technology (ESACT)

Joint Committee Master PBT (“Gemeinsame Kommission Master PBT”)

Examination board Master PBT (“Prüfungsausschuss PBT”)

Admissions Committee Master PBT („Zulassungsausschuss Master PBT“)

Deputy Ombudsperson for Good Scientific Practice (stellv. Ombudsperson für gute wissenschaftliche Praxis)

Publications

Patents

K. Otte, R. Handrick, S. Fischer, "MiRNAs enhancing cell productivity" EU Patent EP2937417B1, issued February 20, 2019.

Peer reviewed publications

1: Herdoíza Padilla E, Crauwels P, Bergner T, Wiederspohn N, Förstner S, Rinas R, Handrick R, Tuckermann J, Otte K, Walther P, Riedel CU. miR-124-5p regulates phagocytosis of human macrophages by targeting the actin cytoskeleton via the ARP2/3 complex. Front. Immunol. 2019 | doi: 10.3389/fimmu.2019.02210.

2: Handl A, López-Lorente ÁI, Handrick R, Mizaikoff B, Hesse F. Infrared attenuated total reflection and 2D fluorescence spectroscopy for the discrimination of differently aggregated monoclonal antibodies. Analyst. 2019 Sep 25. doi: 10.1039/c9an00424f. [Epub ahead of print] PubMed PMID: 31553337.

3: Zeh N, Schneider H, Mathias S, Raab N, Kleemann M, Schmidt-Hertel S, Weis B, Wissing S, Strempel N, Handrick R, Otte K. Human CAP cells represent a novel source for functional, miRNA-loaded exosome production. PLoS One. 2019 Aug 28;14(8):e0221679. doi: 10.1371/journal.pone.0221679. eCollection 2019. PubMed PMID: 31461486; PubMed Central PMCID: PMC6713437.

4: Gauttam R, Seibold GM, Mueller P, Weil T, Weiß T, Handrick R, Eikmanns BJ. A simple dual-inducible CRISPR interference system for multiple gene targeting in Corynebacterium glutamicum. Plasmid. 2019 May;103:25-35. doi: 10.1016/j.plasmid.2019.04.001. Epub 2019 Apr 4. PubMed PMID: 30954454.

5: Raab N, Mathias S, Alt K, Handrick R, Fischer S, Schmieder V, Jadhav V, Borth N, Otte K. CRISPR/Cas9-mediated knockout of microRNA-744 improves antibody titer of CHO production cell lines. Biotechnol J. 2019 Feb 25:e1800477. doi: 10.1002/biot.201800477. [Epub ahead of print] PubMed PMID: 30802343.

6: Kleemann M, Schneider H, Unger K, Bereuther J, Fischer S, Sander P, Marion Schneider E, Fischer-Posovszky P, Riedel CU, Handrick R, Otte K. Induction of apoptosis in ovarian cancer cells by miR-493-3p directly targeting AKT2, STK38L, HMGA2, ETS1 and E2F5. Cell Mol Life Sci. 2019 Feb;76(3):539-559. doi: 10.1007/s00018-018-2958-x. Epub 2018 Nov 3. PubMed PMID: 30392041.

7: Mueller P, Gauttam R, Raab N, Handrick R, Wahl C, Leptihn S, Zorn M, Kussmaul M, Scheffold M, Eikmanns B, Elling L, Gaisser S. High level in vivo mucin-type glycosylation in Escherichia coli. Microb Cell Fact. 2018 Oct 26;17(1):168. doi: 10.1186/s12934-018-1013-9. PubMed PMID: 30367634; PubMed Central PMCID: PMC6202839.

8: Weis BL, Guth N, Fischer S, Wissing S, Fradin S, Holzmann KH, Handrick R, Otte K. Stable miRNA overexpression in human CAP cells: Engineering alternative production systems for advanced manufacturing of biologics using miR-136 and miR-3074. Biotechnol Bioeng. 2018 Aug;115(8):2027-2038. doi: 10.1002/bit.26715. Epub 2018 May 3. PubMed PMID: 29665036.

9: Kleemann M, Schneider H, Unger K, Sander P, Schneider EM, Fischer-Posovszky P, Handrick R, Otte K. MiR-744-5p inducing cell death by directly targeting HNRNPC and NFIX in ovarian cancer cells. Sci Rep. 2018 Jun 13;8(1):9020. doi: 10.1038/s41598-018-27438-6. PubMed PMID: 29899543; PubMed Central PMCID: PMC5998049.

10: Flum M, Kleemann M, Schneider H, Weis B, Fischer S, Handrick R, Otte K. miR-217-5p induces apoptosis by directly targeting PRKCI, BAG3, ITGAV and MAPK1 in colorectal cancer cells. J Cell Commun Signal. 2018 Jun;12(2):451-466. doi: 10.1007/s12079-017-0410-x. Epub 2017 Sep 14. PubMed PMID: 28905214; PubMed Central PMCID: PMC5910322.

11: Paul AJ, Handrick R, Ebert S, Hesse F. Identification of process conditions influencing protein aggregation in Chinese hamster ovary cell culture. Biotechnol Bioeng. 2018 May;115(5):1173-1185. doi: 10.1002/bit.26534. Epub 2018 Jan 22. PubMed PMID: 29280480.

12: Mathias S, Fischer S, Handrick R, Fieder J, Schulz P, Bradl H, Gorr I, Gamer M, Otte K. Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy. J Biotechnol. 2018 Apr 10;271:47-55. doi: 10.1016/j.jbiotec.2018.02.009. Epub 2018 Mar 2. PubMed PMID: 29477386.

13: Paul AJ, Bickel F, Röhm M, Hospach L, Halder B, Rettich N, Handrick R, Herold EM, Kiefer H, Hesse F. High-throughput analysis of sub-visible mAb aggregate particles using automated fluorescence microscopy imaging. Anal Bioanal Chem. 2017 Jul;409(17):4149-4156. doi: 10.1007/s00216-017-0362-2. Epub 2017 Apr 27. PubMed PMID: 28447132.

14: Schoellhorn M, Fischer S, Wagner A, Handrick R, Otte K. miR-143 targets MAPK7 in CHO cells and induces a hyperproductive phenotype to enhance production of difficult-to-express proteins. Biotechnol Prog. 2017 Jul;33(4):1046-1058. doi: 10.1002/btpr.2475. Epub 2017 Apr 21. PubMed PMID: 28371547.

15: Kleemann M, Bereuther J, Fischer S, Marquart K, Hänle S, Unger K, Jendrossek V, Riedel CU, Handrick R, Otte K. Investigation on tissue specific effects of pro-apoptotic micro RNAs revealed miR-147b as a potential biomarker in ovarian cancer prognosis. Oncotarget. 2017 Mar 21;8(12):18773-18791. doi: 10.18632/oncotarget.13095. PubMed PMID: 27821806; PubMed Central PMCID: PMC5386646.

16: Oeck S, Al-Refae K, Riffkin H, Wiel G, Handrick R, Klein D, Iliakis G, Jendrossek V. Activating Akt1 mutations alter DNA double strand break repair and radiosensitivity. Sci Rep. 2017 Feb 17;7:42700. doi: 10.1038/srep42700. PubMed PMID: 28209968; PubMed Central PMCID: PMC5314324.

17: Schwab K, Amann T, Schmid J, Handrick R, Hesse F. Exploring the capabilities of fluorometric online monitoring on chinese hamster ovary cell cultivations producing a monoclonal antibody. Biotechnol Prog. 2016 Nov;32(6):1592-1600. doi: 10.1002/btpr.2326. Epub 2016 Jul 30. PubMed PMID: 27390298.

18: Emmerling VV, Fischer S, Kleemann M, Handrick R, Kochanek S, Otte K. miR-483 is a self-regulating microRNA and can activate its own expression via USF1 in HeLa cells. Int J Biochem Cell Biol. 2016 Nov;80:81-86. doi: 10.1016/j.biocel.2016.09.022. Epub 2016 Sep 28. PubMed PMID: 27693430.

19: Matschke J, Riffkin H, Klein D, Handrick R, Lüdemann L, Metzen E, Shlomi T, Stuschke M, Jendrossek V. Targeted Inhibition of Glutamine-Dependent Glutathione Metabolism Overcomes Death Resistance Induced by Chronic Cycling Hypoxia. Antioxid Redox Signal. 2016 Jul 10;25(2):89-107. doi: 10.1089/ars.2015.6589. Epub 2016 May 9. PubMed PMID: 27021152.

20: Röhm M, Handl A, König M, Mavoungou C, Handrick R, Schindowski K. Data of rational process optimization for the production of a full IgG and its Fab fragment from hybridoma cells. Data Brief. 2016 Jun 2;8:426-35. doi: 10.1016/j.dib.2016.05.067. eCollection 2016 Sep. PubMed PMID: 27366780; PubMed Central PMCID: PMC4910301.

21: Emmerling VV, Fischer S, Stiefel F, Holzmann K, Handrick R, Hesse F, Hörer M, Kochanek S, Otte K. Temperature-sensitive miR-483 is a conserved regulator of recombinant protein and viral vector production in mammalian cells. Biotechnol Bioeng. 2016 Apr;113(4):830-41. doi: 10.1002/bit.25853. Epub 2015 Oct 20. PubMed PMID: 26461143.

22: Fischer S, Handrick R, Otte K. The art of CHO cell engineering: A comprehensive retrospect and future perspectives. Biotechnol Adv. 2015 Dec;33(8):1878-96. doi: 10.1016/j.biotechadv.2015.10.015. Epub 2015 Oct 31.

23: Fischer S, Mathias S, Schaz S, Emmerling VV, Buck T, Kleemann M, Hackl M, Grillari J, Aschrafi A, Handrick R, Otte K. Enhanced protein production by microRNA-30 family in CHO cells is mediated by the modulation of the ubiquitin pathway. J Biotechnol. 2015 Oct 20;212:32-43. doi: 10.1016/j.jbiotec.2015.08.002. Epub 2015 Aug 6. PubMed PMID: 26256096.

24: Fischer S, Paul AJ, Wagner A, Mathias S, Geiss M, Schandock F, Domnowski M, Zimmermann J, Handrick R, Hesse F, Otte K. miR-2861 as novel HDAC5 inhibitor in CHO cells enhances productivity while maintaining product quality. Biotechnol Bioeng. 2015 Oct;112(10):2142-53. doi: 10.1002/bit.25626. Epub 2015 Jun 30. PubMed PMID: 25997799.

25: Paul AJ, Schwab K, Prokoph N, Haas E, Handrick R, Hesse F. Fluorescence dye-based detection of mAb aggregates in CHO culture supernatants. Anal Bioanal Chem. 2015 Jun;407(16):4849-56. doi: 10.1007/s00216-015-8672-8. Epub 2015 Apr 14. PubMed PMID: 25869484.

26: Lauber J, Handrick R, Leptihn S, Dürre P, Gaisser S. Expression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coli. Microb Cell Fact. 2015 Jan 13;14:3. doi: 10.1186/s12934-014-0186-0. PubMed PMID: 25582753; PubMed Central PMCID: PMC4299809.

27: Fischer S, Handrick R, Aschrafi A, Otte K. Unveiling the principle of microRNA-mediated redundancy in cellular pathway regulation. RNA Biol. 2015;12(3):238-47. doi: 10.1080/15476286.2015.1017238. PubMed PMID: 25826657; PubMed Central PMCID: PMC4615785.

28: Fischer S, Buck T, Wagner A, Ehrhart C, Giancaterino J, Mang S, Schad M, Mathias S, Aschrafi A, Handrick R, Otte K. A functional high-content miRNA screen identifies miR-30 family to boost recombinant protein production in CHO cells. Biotechnol J. 2014 Oct;9(10):1279-92. doi: 10.1002/biot.201400306. Epub 2014 Aug 26. PubMed PMID: 25061012.

29: Enlund E, Fischer S, Handrick R, Otte K, Debatin KM, Wabitsch M, Fischer-Posovszky P. Establishment of lipofection for studying miRNA function in human adipocytes. PLoS One. 2014 May 21;9(5):e98023. doi: 10.1371/journal.pone.0098023. eCollection 2014. PubMed PMID: 24849298; PubMed Central PMCID: PMC4029777.

30: Ontikatze T, Rudner J, Handrick R, Belka C, Jendrossek V. Dihydroartemisinin is a Hypoxia-Active Anti-Cancer Drug in Colorectal Carcinoma Cells. Front Oncol. 2014 May 19;4:116. doi: 10.3389/fonc.2014.00116. eCollection 2014. PubMed PMID: 24904829; PubMed Central PMCID: PMC4032948.

31: Fischer S, Wagner A, Kos A, Aschrafi A, Handrick R, Hannemann J, Otte K. Breaking limitations of complex culture media: functional non-viral miRNA delivery into pharmaceutical production cell lines. J Biotechnol. 2013 Dec;168(4):589-600. doi: 10.1016/j.jbiotec.2013.08.027. Epub 2013 Aug 29. PubMed PMID: 23994267.

32: Boubeva R, Reichert C, Handrick R, Müller C, Hannemann J, Borcharda G. New expression method and characterization of recombinant human granulocyte colony stimulating factor in a stable protein formulation. Chimia (Aarau). 2012;66(5):281-5. doi: 10.2533/chimia.2012.281. PubMed PMID: 22867536.

33: Ranta F, Leveringhaus J, Theilig D, Schulz-Raffelt G, Hennige AM, Hildebrand DG, Handrick R, Jendrossek V, Bosch F, Schulze-Osthoff K, Häring HU, Ullrich S. Protein kinase C delta (PKCδ) affects proliferation of insulin-secreting cells by promoting nuclear extrusion of the cell cycle inhibitor p21Cip1/WAF1. PLoS One. 2011;6(12):e28828. doi: 10.1371/journal.pone.0028828. Epub 2011 Dec 27. PubMed PMID: 22216119; PubMed Central PMCID: PMC3246440.

34: Rudner J, Ruiner CE, Handrick R, Eibl HJ, Belka C, Jendrossek V. The Akt-inhibitor Erufosine induces apoptotic cell death in prostate cancer cells and increases the short term effects of ionizing radiation. Radiat Oncol. 2010 Nov 16;5:108. doi: 10.1186/1748-717X-5-108. PubMed PMID: 21080918; PubMed Central PMCID: PMC2998511.

35: Handrick R, Ontikatze T, Bauer KD, Freier F, Rübel A, Dürig J, Belka C, Jendrossek V. Dihydroartemisinin induces apoptosis by a Bak-dependent intrinsic pathway. Mol Cancer Ther. 2010 Sep;9(9):2497-510. doi: 10.1158/1535-7163.MCT-10-0051. Epub 2010 Jul 27. PubMed PMID: 20663933.

36: Rudner J, Mueller AC, Matzner N, Huber SM, Handrick R, Belka C, Jendrossek V. The additional loss of Bak and not the lack of the protein tyrosine kinase p56/Lck in one JCaM1.6 subclone caused pronounced apoptosis resistance in response to stimuli of the intrinsic pathway. Apoptosis. 2009 May;14(5):711-20. doi: 10.1007/s10495-009-0342-x. PubMed PMID: 19343496.

37: Handrick R, Ganswindt U, Faltin H, Goecke B, Daniel PT, Budach W, Belka C, Jendrossek V. Combined action of celecoxib and ionizing radiation in prostate cancer cells is independent of pro-apoptotic Bax. Radiother Oncol. 2009 Mar;90(3):413-21. doi: 10.1016/j.radonc.2008.10.021. Epub 2008 Nov 27. PubMed PMID: 19038466.

38: Müller AC, Handrick R, Elsaesser SJ, Rudner J, Henke G, Ganswindt U, Belka C, Jendrossek V. Importance of Bak for celecoxib-induced apoptosis. Biochem Pharmacol. 2008 Oct 30;76(9):1082-96. doi: 10.1016/j.bcp.2008.08.012. Epub 2008 Aug 19. PubMed PMID: 18778687.

39: Jendrossek V, Henkel M, Hennenlotter J, Vogel U, Ganswindt U, Müller I, Handrick R, Anastasiadis AG, Kuczyk M, Stenzl A, Belka C. Analysis of complex protein kinase B signalling pathways in human prostate cancer samples. BJU Int.2008 Aug;102(3):371-82. doi: 10.1111/j.1464-410X.2008.07703.x. Epub 2008 May 12. PubMed PMID: 18476967.

40: Heinzelmann F, Jendrossek V, Lauber K, Nowak K, Eldh T, Boras R, Handrick R, Henkel M, Martin C, Uhlig S, Köhler D, Eltzschig HK, Wehrmann M, Budach W, Belka C. Irradiation-induced pneumonitis mediated by the CD95/CD95-ligand system. J Natl Cancer Inst. 2006 Sep 6;98(17):1248-51. PubMed PMID: 16954477.

41: Handrick R, Rübel A, Faltin H, Eibl H, Belka C, Jendrossek V. Increased cytotoxicity of ionizing radiation in combination with membrane-targeted apoptosis modulators involves downregulation of protein kinase B/Akt-mediated survival-signaling. Radiother Oncol. 2006 Aug;80(2):199-206. Epub 2006 Aug 17. PubMed PMID: 16916558.

42: Rübel A, Handrick R, Lindner LH, Steiger M, Eibl H, Budach W, Belka C, Jendrossek V. The membrane targeted apoptosis modulators erucylphosphocholine and erucylphosphohomocholine increase the radiation response of human glioblastoma cell lines in vitro. Radiat Oncol. 2006 Mar 29;1:6. PubMed PMID: 16722524; PubMed Central PMCID: PMC1475859.

43: Habermehl D, Kammerer B, Handrick R, Eldh T, Gruber C, Cordes N, Daniel PT, Plasswilm L, Bamberg M, Belka C, Jendrossek V. Proapoptotic activity of Ukrain is based on Chelidonium majus L. alkaloids and mediated via a mitochondrial death pathway. BMC Cancer. 2006 Jan 17;6:14. PubMed PMID: 16417634; PubMed Central PMCID: PMC1379651.

44: Handrick R, Rudner J, Müller I, Eibl H, Belka C, Jendrossek V. Bcl-2 mediated inhibition of erucylphosphocholine-induced apoptosis depends on its subcellular localisation. Biochem Pharmacol. 2005 Sep 15;70(6):837-50. PubMed PMID: 16083863.

45: Schultheiss D, Handrick R, Jendrossek D, Hanzlik M, Schüler D. The presumptive magnetosome protein Mms16 is a poly(3-hydroxybutyrate) granule-bound protein (phasin) in Magnetospirillum gryphiswaldense. J Bacteriol. 2005 Apr;187(7):2416-25. PubMed PMID: 15774885; PubMed Central PMCID: PMC1065218.

46: Handrick R, Reinhardt S, Kimmig P, Jendrossek D. The "intracellular" poly(3-hydroxybutyrate) (PHB) depolymerase of Rhodospirillum rubrum is a periplasm-located protein with specificity for native PHB and with structural similarity to extracellular PHB depolymerases. J Bacteriol. 2004 Nov;186(21):7243-53. PubMed PMID: 15489436; PubMed Central PMCID: PMC523223.

47: Weinmann M, Jendrossek V, Handrick R, Güner D, Goecke B, Belka C. Molecular ordering of hypoxia-induced apoptosis: critical involvement of the mitochondrial death pathway in a FADD/caspase-8 independent manner. Oncogene. 2004 May 6;23(21):3757-69. PubMed PMID: 15034549.

48: Handrick R, Reinhardt S, Schultheiss D, Reichart T, Schüler D, Jendrossek V, Jendrossek D. Unraveling the function of the Rhodospirillum rubrum activator of polyhydroxybutyrate (PHB) degradation: the activator is a PHB-granule-bound protein (phasin). J Bacteriol. 2004 Apr;186(8):2466-75. PubMed PMID: 15060050; PubMed Central PMCID: PMC412128.

49: Handrick R, Technow U, Reichart T, Reinhardt S, Sander T, Jendrossek D. The activator of the Rhodospirillum rubrum PHB depolymerase is a polypeptide that is extremely resistant to high temperature (121 degrees C) and other physical or chemical stresses. FEMS Microbiol Lett. 2004 Jan 30;230(2):265-74. PubMed PMID: 14757249.

50: Jendrossek V, Handrick R. Membrane targeted anticancer drugs: potent inducers of apoptosis and putative radiosensitisers. Curr Med Chem Anticancer Agents. 2003 Sep;3(5):343-53. Review. PubMed PMID: 12871080.

51: Jendrossek V, Handrick R, Belka C. Celecoxib activates a novel mitochondrial apoptosis signaling pathway. FASEB J. 2003 Aug;17(11):1547-9. Epub 2003 Jun 17. PubMed PMID: 12824303.

52: Braaz R, Handrick R, Jendrossek D. Identification and characterisation of the catalytic triad of the alkaliphilic thermotolerant PHA depolymerase PhaZ7 of Paucimonas lemoignei. FEMS Microbiol Lett. 2003 Jul 15;224(1):107-12. PubMed PMID: 12855176.

53: Reinhardt S, Handrick R, Jendrossek D. The "PHB depolymerase inhibitor" of Paucimonas lemoignei is a PHB depolymerase. Biomacromolecules. 2002 Jul-Aug;3(4):823-7. PubMed PMID: 12099828.

54: Jendrossek D, Handrick R. Microbial degradation of polyhydroxyalkanoates. Annu Rev Microbiol. 2002;56:403-32. Epub 2002 Jan 30. Review. PubMed PMID: 12213937.

55: JENDROSSEK, D. and HANDRICK, R. (2001) Diversität bakterieller PHB-Depolymerasen am Beispiel von Paucimonas gen. nov. lemoignei comb. nov. BIOspektrum (6): 521-526.

56: Handrick R, Reinhardt S, Focarete ML, Scandola M, Adamus G, Kowalczuk M, Jendrossek D. A new type of thermoalkalophilic hydrolase of Paucimonas lemoignei with high specificity for amorphous polyesters of short chain-length hydroxyalkanoic acids. J Biol Chem. 2001 Sep 28;276(39):36215-24. Epub 2001 Jul 16. PubMed PMID: 11457823.

57: Handrick R, Reinhardt S, Jendrossek D. Mobilization of poly(3-hydroxybutyrate) in Ralstonia eutropha. J Bacteriol. 2000 Oct;182(20):5916-8. PubMed PMID: 11004196; PubMed Central PMCID: PMC94719.

58. JENDROSSEK, D., SCHIRMER, A. and HANDRICK, R. (1997) Recent advances in charakterization of bacterial PHA depolymerases. In: 1996 International Symphosium on Bacterial Polyhydroxyalkanoates (Eggink, G., Steinbüchel, A., Priorier, Y., Witholt, B., Eds.), Ottawa, Canada: NRC Research Press, 89-101.

59. HANDRICK, R. and JENDROSSEK, D. (1998) Extracellular and intracellular polyhydroxyalkanoate depolymerases: homologies and differences. In: Biochemical Principles and Mechanisms of Biodegradation and Biodegradation of Polymers (Steinbüchel, A., Ed.), Weinheim: Wiley-VCH, 57-67.

Relevant published poster presentations (BMC Proceedings) 

Stützle M,  Moll A,  Handrick R,  Schindowski K. Optimized fermentation conditions for improved antibody yield in hybridoma cells. BMC Proceedings (2013), 7(Suppl 6):P74. 

Relevant application notes

Handrick R, Kozakiewicz F, Doederlein T, Schad M, Riechers D, Otte K, Hannemann, J. (2010) Optimization of monoclonal antibody production employing SENSOLUX® noninvasive sensor technology. Application Note, Sartorius Stedim Biotech.