Cell Culture Technology Laboratory
Component code / course code: (PBT15-ZKT)
Semester: winter / summer
ECTS credits: 6
Lecture hours per week (SWS): 5
Lecturer: Prof. Dr. Jürgen Hannemann
Course language: English
Prerequisite: Recommendation: Lecture Cell Biology, Practical Course Technical Microbiology.
Objectives (Learning Outcome): Students who have successfully completed this module,
- know the principal function of the equipment used in cell biology laboratories (e.g. microscopes, sterile workbenches (laminar flow benches), CO2-Incubators, etc.).
- can carry out sterile processes under a sterile workbench as part of cell culture work.
- know the basic methods for isolating transfected and selected cells (adherent cells and suspension cells).
- know the function of the serum frequently used in cell culture (as a media additive) and the basic functions of the growth factors used.
- have a good basic practical knowledge of standard methods in cell biology laboratories (e.g. trypsinization of adherent cells, cell counting using Neubauer cell counting chamber and automated systems (Cedex), transfection of adherent cells with different transfection reagents, upscaling of suspension cells (hybridoma cells) from T25 flask to shaker flask and roller bottles, analysis of cellular GFP (Green Fluorescent Protein) expression using inverted fluorescence microscope and flow cytometry.
- know the differences when working with adherent cells and cells growing in suspension.
- know different cell lines (adherent and suspension cell lines).
Lecture topics (content):
- Sterile work under a sterile workbench
- Cultivation of cells that grow adherently or in suspension
- Expansion of suspension cells from the T-flask, shaking flask, spinner to roller bottles
- Trypan blue staining and cell counting using the Neubauer counting chamber and the automated cell counting device "Cedex”
- Calculate and set the required cell density for passaging cells
- Different transfection methods
- Analysis of fibroblast cells transfected with the GFP (Green Fluorescent Protein) gene by fluorescence microscope and flow cytometer
- Analysis of in-process controls such as glucose content, pO2, pH, ammonium, lactate.
Teaching format (e.g. online/in person lecture/Seminar/Lab etc.): Laboratory
Examination: Written exam (90 mins) for the module
Bühnenbild: © enot-poloskun/ iStock
