Cell Culture Technology Seminar
Component code / course code: (PBT15-ZKS)
Semester: winter / summer
ECTS credits: 2
Lecture hours per week (SWS): 1
Lecturer: Prof. Dr. Jürgen Hannemann
Course language: English
Prerequisite: Recommendation: Lecture Cell Biology, Practical Course Technical Microbiology.
Objectives (Learning Outcome): Students who have successfully completed this module,
- know the principal function of the equipment used in cell biology laboratories (e.g. microscopes, sterile workbenches (laminar flow benches), CO2-Incubators, etc.).
- can name the advantages and disadvantages of the different transfection methods
- know the use of retroviral vectors for gene transfer and for use as gene therapy medicinal products (ATMP).
- know the basic methods for isolating transfected and selected cells (adherent cells and suspension cells).
- know the function of the serum frequently used in cell culture (as a media additive) and the basic functions of the growth factors used.
- have a good basic theoretical knowledge of standard methods in cell biology laboratories (e.g. trypsinization of adherent cells, cell counting using Neubauer cell counting chamber and automated systems (Cedex), transfection of adherent cells with different transfection reagents, upscaling of suspension cells (hybridoma cells) from T25 flask to shake flask, spinner to 2L benchtop fermenter, analysis of cellular GFP (Green Fluorescent Protein) expression using inverted fluorescence microscope and flow cytometry.
- know the differences when working with adherent cells and cells growing in suspension.
- know different cell lines (adherent and suspension cell lines).
Lecture topics (content):
- History of cell culture technology
- Theory of sterile working techniques
- Sources and types of contamination
- Media and media components
- Laboratory equipment and sterilization
- Cell staining and cell count
- Cultivation vessels and conditions
- Cell types (adherent cells and suspension cells)
- Different transfection and selection methods
- Gene transfer using retroviral vectors and quantification of these recombinant viruses
Teaching format (e.g. online/in person lecture/Seminar/Lab etc.): Seminar
Examination: Written exam (90 mins) (for the module)
Bühnenbild: © enot-poloskun/ iStock
