Downstream Processing
Component code / course code: (PBT14_BAV/BAP)
Semester: winter / summer
ECTS credits: 8
Lecture hours per week (SWS): 8
Lecturer: Prof. Dr. Hans Kiefer
Course language: English
Prerequisite: Lecture "Downstream Processing (Recommended).
Objectives (Learning Outcome): Students who have successfully completed this module
- know the processing of proteins and other biopharmaceutical active substances from different sources
- can describe how their degree of purity is determined, how critical contaminants are detected and removed
- can pick which methods are suitable for different tasks in each case.
- have been given an overview of methods used in the processing of biopharmaceuticals, especially proteins, on a laboratory and industrial scale and can choose the appropriate methods themselves in specific cases.
- Use chromatography and filtration techniques in the laboratory to purify and analyze recombinant proteins from different sources.
- have learned how to use the chromatography system (ÄKTA pure) independently. They can pack columns and check their packing quality.
Lecture topics (content):
- Overview of multi-stage purification processes
- Cell harvesting, preparation of a lysate; centrifugation and microfiltration techniques.
- Chromatography: IEX, SEC, HIC, RPC, AC
- Ultrafiltration, diafiltration, adsorber membranes
- Separation of DNA, viruses, endotoxin, host cell proteins (HCPs) and product-related contaminants
- Special purification techniques: extraction from aqueous multiphase systems, radial flow chromatography, continuous chromatography, precipitation and crystallization
- Design and implementation of PAT (Process Analytical Technologies) and QbD (Quality by Design)
- Set-up and operation of the ÄKTA-pure chromatography systems
Teaching format (e.g. online/in person lecture/Seminar/Lab etc.): Lecture / Lab
Examination: Written examination
