Product Isolation
Component code / course code:
Semester: summer
ECTS credits: 2
Lecture hours per week (SWS): 2
Lecturer: Prof. Dr. Sybille Ebert
Course language: English
Prerequisite:
Objectives (Learning Outcome):
- Master the important principles and methods of Protein purification on laboratory and process scale
- are able to practically process biomolecules and to characterize
- can precipitation processes, protein crystallization, Chromatography and tangential flow filtrations practical carry out
- can biomolecules on the measurement of Characterize enzyme activities
- can determine the purity of protein solutions.
Lecture topics (content):
- Introduction to the processing of biomolecules
- Cell disruption methods
- Basics of chromatography
- Chromatographic separation methods for the separation of Biomolecules (ion exchanger, hydrophobic interaction, mixed mode, affinity, reversed phase and size exclusion)
- Process design for production scale
- Radial and continuous chromatography
- Precipitation and crystallization
- Filtration process
- Two-phase systems for the separation of biomolecules
Teaching format (e.g. online/in person lecture/Seminar/Lab etc.): Lecture
Examination: Written Examination
