Protein Analytics Laboratory
Component code / course code: (PBT14-PAN)
Semester: winter / summer
ECTS credits: 3
Lecture hours per week (SWS): 3
Lecturer: Prof. Dr. Hans Kiefer
Course language: English
Prerequisite: Recommendation: Lecture protein biochemistry, Seminar Protein Analytics (accompanying practical course).
Objectives (Learning Outcome): Students who have successfully completed this module,
- know the processing of proteins and other biopharmaceutical active substances from different sources
- can describe how their degree of purity is determined, how critical contaminants are detected and removed
- can pick which methods are suitable for different tasks in each case.
- have been given an overview of methods used in the processing of biopharmaceuticals, especially proteins, on a laboratory and industrial scale and can choose the appropriate methods themselves in specific cases.
- Use chromatography and filtration techniques in the laboratory to purify and analyze recombinant proteins from different sources.
- have learned how to use the chromatography system (ÄKTA pure) independently. They can pack columns and check their packing quality.
- have learned protein analytical methods theoretically and practically, which can be carried out in biochemical laboratories without expensive technical equipment.
- can independently compile original English-language publications from topics of protein analytics and present them in English. Based on these publications, they have obtained an overview of protein analytical techniques.
Lecture topics (content):
- Purification of lysozyme from chicken egg white by ion exchange chromatography, protein determination by BCA assay, SDS gel electrophoresis, activity determination
- Measurement and optimization of protein stability
- Removal and detection of critical contaminants (endotoxin, DNA, HCPs) from a protein solution
- Measurement of protein-ligand binding, determination of KD and Bmax
Teaching format (e.g. online/in person lecture/Seminar/Lab etc.): Laboratory
Examination: Written exam (60mins)
