Protein Analytics Seminar
Component code / course code: (PBT14-SPA)
Semester: fall / spring
ECTS credits: 1
Lecture hours per week (SWS): 1
Lecturer: Prof. Dr. Hans Kiefer
Course language: English
Prerequisite: Recommendation: Lecture Protein Biochemistry.
Objectives (Learning Outcome): Students who have successfully completed this module,
- know the processing of proteins and other biopharmaceutical active substances from different sources,
- can describe how their degree of purity is determined, how critical contaminants are detected and removed
- can pick which methods are suitable for different tasks in each case.
- have been given an overview of methods used in the processing of biopharmaceuticals, especially proteins, on a laboratory and industrial scale and can choose the appropriate methods themselves in specific cases.
- Use chromatography and filtration techniques in the laboratory to purify and analyze recombinant proteins from different sources.
- have learned how to use the chromatography system (ÄKTA pure) independently. They can pack columns and check their packing quality.
- have learned protein analytical methods theoretically and practically, which can be carried out in biochemical laboratories without expensive technical equipment.
- can independently compile original English-language publications from topics of protein analytics and present them in English. Based on these publications, they have obtained an overview of protein analytical techniques.
Lecture topics (content):
- Protein identification and quantification
- Protein Immunological detection methods
- Analysis of post-translational modifications
- Measurement of protein activity
- Micromethods/Mass spectrometry
- structure analysis
Teaching format (e.g. online/in person lecture/Seminar/Lab etc.): Seminar
Examination: Written exam (60mins)
