Protein Chemistry 

Component code / course code: 

Semester: summer

ECTS credits: 2

Lecture hours per week (SWS): 30

Lecturer: Prof. Dr. Sybille Ebert

Course language: English

Prerequisite: Basic knowledge in Biochemistry.

Objectives (Learning Outcome): Students who have successfully completed this module

  • know the structure of the proteins and the influence
  • certain amino acids on the secondary structure
  • know methods for engineering enzymes (e.g. Directed Evolution, CASTing)
  • master the most important cofactors and
  • Reaction types and mechanisms in which these are involved.

Lecture topics (content): Introduction to protein chemistry
Structure and composition of proteins:
Stereochemistry of the main chain, structure and mobility of the
side chain, acid-base behavior of the side chains,
Side chain polarity, chemical differentiation
Structural systems in proteins (helix, sheet, turning point,
domains), X-ray structure analysis, interaction
between protein side chains
Non-protein structural components (glycosylation,
phosphate groups, N-terminal acyl residues)
Enzyme screening and protein engineering (rational design,
directed evolution, saturation mutagenesis (CAST, B-Fit)
Coenzymes and reaction mechanisms

Teaching format (e.g. online/in person lecture/Seminar/Lab etc.): Lecture

Examination: Written examination