Protein Chemistry
Component code / course code:
Semester: summer
ECTS credits: 2
Lecture hours per week (SWS): 30
Lecturer: Prof. Dr. Sybille Ebert
Course language: English
Prerequisite: Basic knowledge in Biochemistry.
Objectives (Learning Outcome): Students who have successfully completed this module
- know the structure of the proteins and the influence
- certain amino acids on the secondary structure
- know methods for engineering enzymes (e.g. Directed Evolution, CASTing)
- master the most important cofactors and
- Reaction types and mechanisms in which these are involved.
Lecture topics (content): Introduction to protein chemistry
Structure and composition of proteins:
Stereochemistry of the main chain, structure and mobility of the
side chain, acid-base behavior of the side chains,
Side chain polarity, chemical differentiation
Structural systems in proteins (helix, sheet, turning point,
domains), X-ray structure analysis, interaction
between protein side chains
Non-protein structural components (glycosylation,
phosphate groups, N-terminal acyl residues)
Enzyme screening and protein engineering (rational design,
directed evolution, saturation mutagenesis (CAST, B-Fit)
Coenzymes and reaction mechanisms
Teaching format (e.g. online/in person lecture/Seminar/Lab etc.): Lecture
Examination: Written examination
