Research group Kerstin Otte

Institute members

Team members

Masterands

Laura Starcke

Berenike Walter

Hannah Fahrion

2022

Scientific publications

• Exploring synthetic biology for the development of a sensor cell line for automated bioprocess control. Zeh N, Bräuer M, Raab N, Handrick R, Otte K. Sci Rep. 2022 Feb 10;12(1):2268. doi: 10.1038/s41598-022-06272-x. PMID: 35145179 Free PMC article.
   
• High-throughput glycosylation analysis of intact monoclonal antibodies by mass spectrometry coupled with capillary electrophoresis and liquid chromatography. Naumann L, Schlossbauer P, Klingler F, Hesse F, Otte K, Neusüß C. J Sep Sci. 2022 Jan 19. doi: 10.1002/jssc.202100865. Online ahead of print. PMID: 35044720
   
• Loss of a newly discovered microRNA in Chinese hamster ovary cells leads to upregulation of N-glycolylneuraminic acid sialylation on monoclonal antibodies. Fischer S, Mathias S, Stadermann A, Yang S, Schmieder V, Zeh N, Schmidt N, Richter P, Wright S, Zimmermann E, Ley Y, van der Meer J, Hartsch T, Bernloehr C, Otte K, Bradl H, Gamer M, Schulz P. Biotechnol Bioeng. 2022 Mar;119(3):832-844. doi: 10.1002/bit.28015. Epub 2022 Jan 14. PMID: 34935124
   
• A blueprint from nature: miRNome comparison of plasma cells and CHO cells to optimize therapeutic antibody production. Raab N, Zeh N, Schlossbauer P, Mathias S, Lindner B, Stadermann A, Gamer M, Fischer S, Holzmann K, Handrick R, Otte K. N Biotechnol. 2022 Jan 25;66:79-88. doi: 10.1016/j.nbt.2021.10.005. Epub 2021 Oct 25. PMID: 34710621 Free article.

2021

Scientific publications

• Cell line development for continuous high cell density biomanufacturing: Exploiting hypoxia for improved productivity. Zeh N, Schlossbauer P, Raab N, Klingler F, Handrick R, Otte K. Metab Eng Commun. 2021 Jul 29;13:e00181. doi: 10.1016/j.mec.2021.e00181. eCollection 2021 Dec. PMID: 34401326
   
• Klingler F, Raab N, Zeh N, Otte K (2021). Next Generation Cell Engineering Using microRNAs In: Cell Culture Engineering and Technology: 71- 94, Editor: Ralf Pörtner, ISBN: 978-3-030-79870-3
   
• Exploring the molecular content of CHO exosomes during bioprocessing. Keysberg C, Hertel O, Schelletter L, Busche T, Sochart C, Kalinowski J, Hoffrogge R, Otte K, Noll T. Appl Microbiol Biotechnol. 2021 May;105(9):3673-3689. doi: 10.1007/s00253-021-11309-8. Epub 2021 May 3.
   
• Unveiling the CHO surfaceome: Identification of cell surface proteins reveals cell aggregation-relevant mechanisms.Klingler F, Mathias S, Schneider H, Buck T, Raab N, Zeh N, Shieh YW, Pfannstiel J, Otte K. Biotechnol Bioeng. 2021 Aug;118(8):3015-3028. doi: 10.1002/bit.27811. Epub 2021 May 14. PMID: 33951178
   
• Klingler F, Otte K (2021). Life at the periphery: How cell surface proteins can influence bioprocesses. Pharmind, 83 (9), 1241-1244.
   
•  A blueprint from nature: miRNome comparison of plasma cells and CHO cells to optimize therapeutic antibody production. Raab N, Zeh N, Schlossbauer P, Mathias S, Lindner B, Stadermann A, Gamer M, Fischer S, Holzmann K, Handrick R,Otte K. N Biotechnol. 2021 Oct 25:S1871-6784(21)00094-7. doi: 10.1016/j.nbt.2021.10.005. Online ahead of print.
   
• Cell line development for continuous high cell density biomanufacturing: Exploiting hypoxia for improved productivity. Zeh N, Schlossbauer P, Raab N, Klingler F, Handrick R, Otte K. Metab Eng Commun. 2021 Jul 29;13:e00181. doi: 10.1016/j.mec.2021.e00181. eCollection 2021 Dec. PMID: 34401326

2020

Scientific publications

• Unraveling what makes a monoclonal antibody difficult-to-express: From intracellular accumulation to incomplete folding and degradation via ERAD. Mathias S, Wippermann A, Raab N, Zeh N, Handrick R, Gorr I, Schulz P, Fischer S, Gamer M, Otte K.Biotechnol Bioeng. 2020 Jan;117(1):5-16. doi: 10.1002/bit.27196. Epub 2019 Nov 15. PMID: 31631329
   

• Rational optimization of a monoclonal antibody improves the aggregation propensity and enhances the CMC properties along the entire pharmaceutical process chain. Bauer J, Mathias S, Kube S, Otte K, Garidel P, Gamer M, Blech M, Fischer S, Karow-Zwick AR. MAbs. 2020 Jan-Dec;12(1):1787121. doi: 10.1080/19420862.2020.1787121. PMID: 32658605 Free PMC article.
   
• Transferability of miRNA-technology to bioprocessing: Influence of cultivation mode and media.
  Leroux AC, Bartels E, Winter L, Mann M, Otte K, Zehe C. Biotechnol Prog. 2021 Mar;37(2):e3107. doi: 10.1002/btpr.3107. Epub 2020 Dec 30. PMID: 33300297
   
• Rational optimization of a monoclonal antibody improves the aggregation propensity and enhances the CMC properties along the entire pharmaceutical process chain.
  Bauer J, Mathias S, Kube S, Otte K, Garidel P, Gamer M, Blech M, Fischer S, Karow-Zwick AR. MAbs. 2020 Jan-Dec;12(1):1787121. doi: 10.1080/19420862.2020.1787121. PMID: 32658605 Free PMC article.

2019

Scientific publications

• mir-124-5p Regulates Phagocytosis of Human Macrophages by Targeting the Actin Cytoskeleton via the ARP2/3 Complex.
  Herdoiza Padilla E, Crauwels P, Bergner T, Wiederspohn N, Förstner S, Rinas R, Ruf A, Kleemann M, Handrick R, Tuckermann J, Otte K, Walther P, Riedel CU. Front Immunol. 2019 Oct 4;10:2210. doi: 10.3389/fimmu.2019.02210. eCollection 2019. PMID: 31636629 Free PMC article.
   
• Unraveling what makes a monoclonal antibody difficult-to-express: From intracellular accumulation to incomplete folding and degradation via ERAD.
  Mathias S, Wippermann A, Raab N, Zeh N, Handrick R, Gorr I, Schulz P, Fischer S, Gamer M, Otte K. Biotechnol Bioeng. 2020 Jan;117(1):5-16. doi: 10.1002/bit.27196. Epub 2019 Nov 15. PMID: 31631329
   
• Human CAP cells represent a novel source for functional, miRNA-loaded exosome production.
  Zeh N, Schneider H, Mathias S, Raab N, Kleemann M, Schmidt-Hertel S, Weis B, Wissing S, Strempel N, Handrick R, Otte K. PLoS One. 2019 Aug 28;14(8):e0221679. doi: 10.1371/journal.pone.0221679. eCollection 2019. PMID: 31461486 Free PMC article.
   
• CRISPR/Cas9-Mediated Knockout of MicroRNA-744 Improves Antibody Titer of CHO Production Cell Lines.
  Raab N, Mathias S, Alt K, Handrick R, Fischer S, Schmieder V, Jadhav V, Borth N, Otte K. Biotechnol J. 2019 May;14(5):e1800477. doi: 10.1002/biot.201800477. Epub 2019 Apr 15. PMID: 30802343
   
• Induction of apoptosis in ovarian cancer cells by miR-493-3p directly targeting AKT2, STK38L, HMGA2, ETS1 and E2F5.
  Kleemann M, Schneider H, Unger K, Bereuther J, Fischer S, Sander P, Marion Schneider E, Fischer-Posovszky P, Riedel CU, Handrick R, Otte K. Cell Mol Life Sci. 2019 Feb;76(3):539-559. doi: 10.1007/s00018-018-2958-x. Epub 2018 Nov 3. PMID: 30392041

Invited conference presentations

• Kerstin Otte, Difficult to express proteins:
  Identification of intracellular production bottlenecks in CHO cells
  Bioprocess Summit Europe, Lisbon (Portugal), 19.-21.3.2019
   
• Kerstin Otte, Forschung gemeinsam mit Unternehmen
  Forschung an HAW, Bernhäuser Forst, 28.-29.3.2019
   
• Kerstin Otte, Engineering of mammalian cell lines to remove production bottlenecks
  RPP10 A comparative view on host physiology, Kreta (Griechenland), 24.-27.4.2019
   
• Kerstin Otte, Molecular Modulation of Cellular Signaling to Enhance protein production
  Gordon Research Conference Biotherapeutics and Vaccines Development, Houston (USA), 6.-11.1.2019

2018

Scientific publications

• MiR-744-5p inducing cell death by directly targeting HNRNPC and NFIX in ovarian cancer cells.
  Kleemann M, Schneider H, Unger K, Sander P, Schneider EM, Fischer-Posovszky P, Handrick R, Otte K. Sci Rep. 2018 Jun 13;8(1):9020. doi: 10.1038/s41598-018-27438-6. PMID: 29899543 Free PMC article.
   
• Stable miRNA overexpression in human CAP cells: Engineering alternative production systems for advanced manufacturing of biologics using miR-136 and miR-3074.
  Weis BL, Guth N, Fischer S, Wissing S, Fradin S, Holzmann KH, Handrick R, Otte K. Biotechnol Bioeng. 2018 Aug;115(8):2027-2038. doi: 10.1002/bit.26715. Epub 2018 May 3. PMID: 29665036
   
• Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy.
  Mathias S, Fischer S, Handrick R, Fieder J, Schulz P, Bradl H, Gorr I, Gamer M, Otte K. J Biotechnol. 2018 Apr 10;271:47-55. doi: 10.1016/j.jbiotec.2018.02.009. Epub 2018 Mar 2. PMID: 29477386

Invited conference presentations

• Kerstin Otte, CRISPR/CAS9 mediated knockout of microRNAs for precise cell engineering
  Cell Culture Engineering (CCE) XVI, Tampa (USA), 6-11.5.2018
   
• Kerstin Otte, CRISPR/CAS9 MEDIATED KNOCKOUT OF MICRORNAS FOR PECISE CELL ENGINEERING
  Cell Culture Engineering (CCE) XVI, Tampa (USA), 6-11.5.2018
   
• Kerstin Otte, Identification of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy PEGS Boston 2018, Boston (USA), 30.4.-4.5.2018
   
• Kerstin Otte, Latest developments on MicroRNAs for Next-Generation Cell Engineering 11th Annual Proteins & Antibodies Congress, London (UK), 16-18.4.2018
  Kerstin Otte, ENGINEERING OF EXOSOMES FOR TARGETED DELIVERY OF THERAPEUTIC MICRORNAS
  BPI WEST, San Francisco (USA), 19-22.3.2018

2017

Scientific publications

• miR-217-5p induces apoptosis by directly targeting PRKCI, BAG3, ITGAV and MAPK1 in colorectal cancer cells.
  Flum M, Kleemann M, Schneider H, Weis B, Fischer S, Handrick R, Otte K. J Cell Commun Signal. 2018 Jun;12(2):451-466. doi: 10.1007/s12079-017-0410-x. Epub 2017 Sep 14. PMID: 28905214 Free PMC article.
   
• miR-143 targets MAPK7 in CHO cells and induces a hyperproductive phenotype to enhance production of difficult-to-express proteins.
  Schoellhorn M, Fischer S, Wagner A, Handrick R, Otte K. Biotechnol Prog. 2017 Jul;33(4):1046-1058. doi: 10.1002/btpr.2475. Epub 2017 Apr 21. PMID: 28371547
   
• Investigation on tissue specific effects of pro-apoptotic micro RNAs revealed miR-147b as a potential biomarker in ovarian cancer prognosis.
  Kleemann M, Bereuther J, Fischer S, Marquart K, Hänle S, Unger K, Jendrossek V, Riedel CU, Handrick R, Otte K. Oncotarget. 2017 Mar 21;8(12):18773-18791. doi: 10.18632/oncotarget.13095. PMID: 27821806 Free PMC article.
   

Invited conference presentations

• Kerstin Otte, Effective MicroRNAs for Next-Generation CHO Cell Engineering The 2nd International Advanced Biomanufacturing Conference, Sheffield (UK), 22-23.5.2017
   
• Kerstin Otte, The latest research findings on engineering cell lines by using
  MicroRNAs Bioprocess International European Summit, Cell Line Development & Engineering, Amsterdam (Netherlands), 24.-27.4.2017
   
• Kerstin Otte, MicroRNAs for optimized production: towards a deeper understanding
  of underlying mechanisms Cell Culture and Downstream World Congress 2017, Munich (Germany), 16.1.2017

2016

Scientific publications

• miR-483 is a self-regulating microRNA and can activate its own expression via USF1 in HeLa cells.
  Emmerling VV, Fischer S, Kleemann M, Handrick R, Kochanek S, Otte K. Int J Biochem Cell Biol. 2016 Nov;80:81-86. doi: 10.1016/j.biocel.2016.09.022. Epub 2016 Sep 28. PMID: 27693430
   
• miRNA profiling of high, low and non-producing CHO cells during biphasic fed-batch cultivation reveals process relevant targets for host cell engineering.
  Stiefel F, Fischer S, Sczyrba A, Otte K, Hesse F. J Biotechnol. 2016 May 10;225:31-43. doi: 10.1016/j.jbiotec.2016.03.028. Epub 2016 Mar 19. PMID: 27002234
   
• GATA4 Regulates Blood-Testis Barrier Function and Lactate Metabolism in Mouse Sertoli Cells.
  Schrade A, Kyrönlahti A, Akinrinade O, Pihlajoki M, Fischer S, Rodriguez VM, Otte K, Velagapudi V, Toppari J, Wilson DB, Heikinheimo M. Endocrinology. 2016 Jun;157(6):2416-31. doi: 10.1210/en.2015-1927. Epub 2016 Mar 14. PMID: 26974005 Free PMC article.

Invited conference presentations

• Kerstin Otte, Effective microRNAs for cell line engineering to enhance productivity
  ACTIP meeting, Frankfurt (Germany), 1-2.12.2016
   
• Kerstin Otte, Carriers in Academia
  1st ESACT Frontiers Retreat 2016, Lyon (France), 20-22.10.2016
   
• Kerstin Otte, Effective miRNAs for cell line engineering and underlying cellular mechanisms of action.

2015

Scientific publications

• Three-dimensional tumor spheroids for in vitro analysis of bacteria as gene delivery vectors in tumor therapy.
  Osswald A, Sun Z, Grimm V, Ampem G, Riegel K, Westendorf AM, Sommergruber W, Otte K, Dürre P, Riedel CU. Microb Cell Fact. 2015 Dec 12;14:199. doi: 10.1186/s12934-015-0383-5. PMID: 26655167 Free PMC article.
   
• The art of CHO cell engineering: A comprehensive retrospect and future perspectives.
  Fischer S, Handrick R, Otte K. Biotechnol Adv. 2015 Dec;33(8):1878-96. doi: 10.1016/j.biotechadv.2015.10.015. Epub 2015 Oct 31. PMID: 26523782 Review.
   
• Temperature-sensitive miR-483 is a conserved regulator of recombinant protein and viral vector production in mammalian cells.
  Emmerling VV, Fischer S, Stiefel F, Holzmann K, Handrick R, Hesse F, Hörer M, Kochanek S, Otte K. Biotechnol Bioeng. 2016 Apr;113(4):830-41. doi: 10.1002/bit.25853. Epub 2015 Oct 20. PMID: 26461143
   
• Enhanced protein production by microRNA-30 family in CHO cells is mediated by the modulation of the ubiquitin pathway.
  Fischer S, Mathias S, Schaz S, Emmerling VV, Buck T, Kleemann M, Hackl M, Grillari J, Aschrafi A, Handrick R, Otte K. J Biotechnol. 2015 Oct 20;212:32-43. doi: 10.1016/j.jbiotec.2015.08.002. Epub 2015 Aug 6. PMID: 26256096
   
• miR-2861 as novel HDAC5 inhibitor in CHO cells enhances productivity while maintaining product quality.
  Fischer S, Paul AJ, Wagner A, Mathias S, Geiss M, Schandock F, Domnowski M, Zimmermann J, Handrick R, Hesse F, Otte K. Biotechnol Bioeng. 2015 Oct;112(10):2142-53. doi: 10.1002/bit.25626. Epub 2015 Jun 30. PMID: 25997799
   
• Unveiling the principle of microRNA-mediated redundancy in cellular pathway regulation.
  Fischer S, Handrick R, Aschrafi A, Otte K. RNA Biol. 2015;12(3):238-47. doi: 10.1080/15476286.2015.1017238. PMID: 25826657 Free PMC article.

Invited conference presentations

• Kerstin Otte, Scale-Down Models Enable Identication of microRNAs to Enhance
  Cellular Performance of Mammalian Cell Factories
  PEGS Europe, Lissabon (Spain), 6.11.2015
   
• Kerstin Otte, Tiny but Mighty - Harnessing microRNAs for Next-generation Cell
  Engineering of Biopharmaceutical Production Cells
  3rd Protein Expression, Purification and Characterization Conference, Boston (USA), 23.10.2015
   
• Kerstin Otte, Cell line Engineering to Boost Biopharmaceutical Production.
  8th Student Symposium on Molecular Medicine, Ulm University, 18.4.2015
   
• Kerstin Otte, Werdegang Professorin
  Jubiläumsveranstaltung der LaKof BW, Hochschule Esslingen (Germany), 10.3.2015

2014

Scientific publications

• A functional high-content miRNA screen identifies miR-30 family to boost recombinant protein production in CHO cells.
  Fischer S, Buck T, Wagner A, Ehrhart C, Giancaterino J, Mang S, Schad M, Mathias S, Aschrafi A, Handrick R, Otte K. Biotechnol J. 2014 Oct;9(10):1279-92. doi: 10.1002/biot.201400306. Epub 2014 Aug 26. PMID: 25061012
   
• Establishment of lipofection for studying miRNA function in human adipocytes.
  Enlund E, Fischer S, Handrick R, Otte K, Debatin KM, Wabitsch M, Fischer-Posovszky P. PLoS One. 2014 May 21;9(5):e98023. doi: 10.1371/journal.pone.0098023. eCollection 2014. PMID: 24849298 Free PMC article.
   

Invited conference presentations

• Kerstin Otte, Cell Line Engineering Using the Potential of microRNAs
  PEGS Europe, Lisbon (Portugal), 6.11.2014
   
• Kerstin Otte, The potential of miRNAs in genetic engineering of CHO cells
  MipTec, Basel (Swizerland), 25.10.2014

2013

Scientific publications

• Breaking limitations of complex culture media: functional non-viral miRNA delivery into pharmaceutical production cell lines.
  Fischer S, Wagner A, Kos A, Aschrafi A, Handrick R, Hannemann J, Otte K. J Biotechnol. 2013 Dec;168(4):589-600. doi: 10.1016/j.jbiotec.2013.08.027. Epub 2013 Aug 29. PMID: 23994267
   
• Otte K (2013) Fachkräfte für die Biotechnologiebranche. Ausbildungssituation am
  Standort Deutschland. Pharmind, 11, 1738 - 1742

2004 and older

Scientific publications

• Identification of farnesoid X receptor beta as a novel mammalian nuclear receptor sensing lanosterol.
  Otte K, Kranz H, Kober I, Thompson P, Hoefer M, Haubold B, Remmel B, Voss H, Kaiser C, Albers M, Cheruvallath Z, Jackson D, Casari G, Koegl M, Pääbo S, Mous J, Kremoser C, Deuschle U. Mol Cell Biol. 2003 Feb;23(3):864-72. doi: 10.1128/mcb.23.3.864-872.2003. PMID: 12529392 Free PMC article.
   
• Transcriptional regulation and biological significance of the insulin like growth factor II gene.
  Engström W, Shokrai A, Otte K, Granérus M, Gessbo A, Bierke P, Madej A, Sjölund M, Ward A. Cell Prolif. 1998 Oct-Dec;31(5-6):173-89. doi: 10.1111/j.1365-2184.1998.tb01196.x. PMID: 9925986 Free PMC article. Review.
   
• A conserved structural element in horse and mouse IGF2 genes binds a methylation sensitive factor.
  Otte K, Choudhury D, Charalambous M, Engström W, Rozell B. Nucleic Acids Res. 1998 Apr 1;26(7):1605-12. doi: 10.1093/nar/26.7.1605. PMID: 9512529 Free PMC article.
   
• FISH mapping of the IGF2 gene in horse and donkey-detection of homoeology with HSA11.
  Raudsepp T, Otte K, Rozell B, Chowdhary BP. Mamm Genome. 1997 Aug;8(8):569-72. doi: 10.1007/s003359900505. PMID: 9250862
   
• Cloning and sequencing of an equine insulin-like growth factor I cDNA and its expression in fetal and adult tissues.
  Otte K, Rozell B, Gessbo A, Engström W. Gen Comp Endocrinol. 1996 Apr;102(1):11-5. doi: 10.1006/gcen.1996.0040. PMID: 8860303
   
• Insulin-like growth factor II in the horse: determination of a cDNA nucleotide sequence and expression in fetal and adult tissue.
  Otte K, Engström W. Gen Comp Endocrinol. 1994 Nov;96(2):270-5. doi: 10.1006/gcen.1994.1182. PMID: 785172

• Identification and characterization of production bottlenecks in CHO cells
• Increased bioassay productivity and quality by standardization and automation (Automation
• Crossing Barriers with bio-based therapeutics
• CAP-Exsosomes
• Entwicklung von Hochproduktionszelllinien für ressourcenschonende Herstellung von Biopharmazeutika
• KPK-PBT: Kooperatives Promotionskolleg Pharmazeutische Biotechnologie